To resolve the issue, you should grant Full Control permission to ASPNET, NETWORK SERVICE and Internet Guest Account system accounts over App_Data folder:
1. In Windows Explorer, go to the folder, right-click on App_Data folder and choose Properties:
2. In General tab, make sure "Read-only" option is not set:
3. In Security tab, gran "Full Control" permission to ASPNET, NETWORK SERVICE and Internet Guest Account accounts:
4. Click OK. Now, ASP.NET should have enough permissions to read/write files and folders in the App_Data folder.
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